Bautista connected for a two-run blast - his first bomb of the Spring - off Astro starter Zack Duke in the third inning and sent the first pitch he saw from right-hander Jordan Lyles over the 400-foot sign in right center field in the fifth.
In 2011, Bautista slammed 43 homers to lead the American League for the second straight season, driving in 103 runs for the Jays. The slugging right-fielder had 54 in 2010 with 124 RBI.
Barry Zito worked three scoreless innings for his second win of the exhibition season, and promising rookie prospect Tommy Joseph belted a pair of home runs, driving in four runs in three at-bats.
Joseph, who has never played in a major league game, is batting .444 (4-for-9) in four games thus far and has driven in six runs. Having played high school ball in Scottsdale, 21-year-old Joseph thrilled the locals with his performance on Saturday. He played with Class A Advanced San Jose last season, ripping 22 home runs and 95 RBI while being named to California League postseason All-Star team.